The research on the protein blotting was fruitful during the past few years. This review summarizes the literature relating to the application of protein blotting in the fields as following:solid-phase sequence analysis of proteins, clinical differential diagnosis of various virulence antigens or antibodies in human serum or urine,capillary column chromatography and preparative capillary electrophoresis of macromolecular biomaterials,purification of monoclone antibodies or monoepitopic antibodies,and detection of proteins and enzymes using various antibodies or ligands.