Five ligands containing benzimidazolyl and 32 compounds of their containing copper (Ⅱ), iron(Ⅲ), manganese (Ⅱ), cobalt (Ⅱ) have been synthesized based on the active site structures of natural superoxide dismutase (SOD), respectively. It has been shown that these compounds possess mimetic SOD activities by spectroscopic and electrochemical measurements. The concentrations of 50% inhibition (IC50) are within 10-6～10-8mol·L-1. The rate constants (kq) of catalytic dismutation of superoxide radicals (O-·2) are in the range of 106～108mol-1·L·s-1.Simultaneously it has been observed that a several mimetic compounds of then have antineoplastic activities and increasing antifreezing effect of rices.