A cDNA library of Wistar rat brain was constructed with a highly effcient and simplified method. The template Poly (A)⁺-RNA was extracted from Wistar rat brain. The first strand of cDNA was synthesized by SuperScript RNase H^- reverse transcriptase with the oligo (dT)₁₅ primer which contains Not I site. After the poly (A)⁺ -RNA was removed with E. coli RNase H, the second strand was synthesized by means of E. coli DNA polymerase I, E. coli DNA ligase and T4 DNA polymerase.Then Sal I adapter was added and the cDNA digested with Not I .Half of the cDNA was inserted into plasmid pSPORT I and transformed E .coli DH 5a. The other half was stored at -20℃. It was shown that the cDNA length ranged from 100 to 10 000 bp. Using this cDNA library as a template, four genes have been amplified successfully. They are glutamic acid decarboxylase (GAD, 1800 bp)，neuron-specific enolase (NSE, 1340 bp)，T3 receptor(1230 bp) and cholecystokinin(CCK, 345 bp).The method for storage of the cDNA library which can be kept for a longer period was also developed.