分离及纯化兔肝金属硫蛋白.制备去金属金属硫蛋白、锌7与镉7金属硫蛋白.在不同pH条件下,比较后二者清除羟自由基能力;在pH6条件下,比较锌7-金属硫蛋白与有关蛋白和无机锌盐清除羟自由基效果.结论是在近生理pH条件下锌7-金属硫蛋白清除羟自由基能力远强于镉7-金属硫蛋白.金属硫蛋白清除羟自由基的能力主要来源于蛋白中处于还原态的流基.
Metallothionein (rabbit liver)(MT),apo-MT, Zn7-MT and Cd7-MT were prepared respectively. The scavenging effects of Zn7-MT and Cd7-MT on hydroxyl radical were compared at various pH values, and at pH 6 the effects of Zn7-MT were compared with related proteins and inorganic zinc salt. From these results it is concluded that the ability of Zn7-MT to scavenge hydroxyl radical is far stronger than that of Cd7MT and that the ability of MT to scavenge hydroxyl radical derives from reduced -SH group in MT.
岳新萍,周杰昊,程时.锌7与镉7-金属硫蛋白清除羟自由基的比较[J].生物化学与生物物理进展,1996,23(4):352-355
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