介绍一种鉴别SOD类型的方法─—聚丙烯酰胺凝胶电泳的定位染色法. 由于不同类型的SOD对抑制剂的表现各异, 电泳后的凝胶经不同的抑制剂处理, 染色, 结果展示在凝胶上, CuZn-SOD酶带在H2O2或CN-的作用下消失, Mn-SOD在CHCl3-CH3OH作用下消失, Fe-SOD在H2O2或. CHCl3-CH3CH2OH作用下失活, 从酶带消失或存活的情况, 可以判断SOD的类型.
A simple method, the location staining method, for distinguishing different SOD types was introduced. Due to the different performances of the different types of SOD against inhibitors, .e. g. Cu, Zn-SOD band disappeared in the presence of H2O2 or CN-, MnSOD disappeared in the presence of CHCl3CH3CH2OH, and Fe-SOD lost in the presence of H2O2 or CHCl3-CH3CH2OH on the electrophoresis gels, the gels after electrophoresis were treated with the different inhibitors and then stained. According to the loss or presence of these bands, different SOD types can be identified.
罗广华,王爱国,付爱根.鉴别超氧化物歧化酶类型的定位染色法[J].生物化学与生物物理进展,1996,23(4):356-359
复制生物化学与生物物理进展 ® 2025 版权所有 ICP:京ICP备05023138号-1 京公网安备 11010502031771号