国家“863”高科技发展计划、攀登计划、博士后基金及World Lab资助项目.
滤纸条半干技术在保证分辨率的前提下, 将凝胶电泳的电泳时间从原来的2~4h缩短到40~50min, 并简化了操作, 节省了实验费用, 不需配制大量的缓冲液. pH4.8较pH8.9阳极电泳提高了酸性蛋白质的电泳分辨率. pH5.5阴极电泳用于分离碱性样品可以得到很好的效果.
Rapid semi-dry technique by filter strips shortens running time from 2~4h to 40~50 min without lowering resolution and using large amount of electrode buffer.It also simplifies operation and saves materials. Being compared with pH 8.9 anode PAGE, pH 4.8 PAGE has higher resolution in acid protein seperation.Very good results can be obtained by using pH 5.5 cathode PAGE with semi-dry technique by filter strips for alkaline sample separations.
郭尧君,余添.阳极电泳和阴极电泳的快速半干新技术[J].生物化学与生物物理进展,1996,23(4):359-364
复制生物化学与生物物理进展 ® 2025 版权所有 ICP:京ICP备05023138号-1 京公网安备 11010502031771号