应用酸性、中性交互提取法从人胚骨中提取Ⅰ型胶原,经SDS-PAGE电泳,氨基酸分析和免疫学方法鉴定.结果表明所提胶原电泳区带与Ⅰ型标准品相同,环状沉淀反应阳性,氨基酸分析甘氨酸占1000个氨基酸总残基的1/3,羟脯氨酸与脯氨酸之比为0.65,符合Ⅰ型胶原特征,并显示有较高的纯度,可用于胶原制品的制作.
Type Ⅰ collagen was extracted from human embryonal bone by alternating acidic and neutral salt precipitation technique and was identified by SDS-PAGE, amino acid analysis and immunological method. The results showed that electrophoresis band of collagen extracted was the same as that of type Ⅰ collagen standard, ring precipitation test was positive, glycine was 1/3 in total of 1000 amino acid residues, the ratio of hydroxyproline and proline was 0.65. These suggested that the extracted collagen accord with the characteristics of type Ⅰ collagen and is highly purified. It could be used to prepare collagen products.
李成章,樊明文.胚胎骨Ⅰ型胶原的提取与鉴定[J].生物化学与生物物理进展,1996,23(4):373-375
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