Resistance of HeLa Line Irradiated with 60Co γ-Ray to the Interstrand DNA Cross-link Induced by Diamminedichloroplatinum
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摘要:
应用EB荧光分析法测定了人宫颈癌上皮细胞系(HeLa line)经单次 60Co照射后顺铂(CDDP)诱导的DNA链内交联反应(ct％).经研究发现，1～6 Gy照射后，细胞相对生长分数(fraction of control growth，FCG) 减小，单位细胞DNA含量增高，HeLa细胞与CDDP的交联反应明显降低，且与照射剂量呈依赖关系.结果提示，γ射线照射后存活的肿瘤细胞可能对CDDP或烷化剂的化学治疗作用产生抗性.
Abstract:
The detection of DNA cross-link induced by diaminedichloroplatinum (CDDP) in HeLa line irradiated with 60Co γ-ray was carried out using ethidium bromide fluorescence assay. The results showed that after single doses (1～6 Gy)of irradiation, the fraction of control growth of HeLa cells declined and the DNA content per unit cell of HeLa line increased, and the formation of interstrand DNA cross-link induced by CDDP reduced apparently with increments of dose of irradiation. The findings suggest that tumor cells surviving γ-irradiation may become resistant to chemotherapy with alkylating agents such as CDDP.
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