湖北省95重点科技攻关计划项目资助课题(96192004)
以人胚胎mRNA为模板,采用RT-PCR法得到了人补体调节蛋白膜辅蛋白(MCP)的一种cDNA全基因,序列分析结果表明,所获得的MCP cDNA为文献报道中10种同种型中的一种,属MCP-C2型,该cDNA含一编码369个氨基酸的阅读框架,其中的STP区含14个氨基酸,由STPC编码,胞浆尾区含23个氨基酸,为CYT2,未发现有东西方人种之间的核苷酸的差异.
MCP is an important membrane protein for protecting host cells from damage by complement because of its wide tissue distribution and cofactor activity. A human MCP cDNA containing full encoding region was obtained by RT-PCR from Chinese human embryo. The target gene was cloned and sequenced. The data indicated that there are forty-five bases deletion compared with the counterparts previouly reported,which leads to the deletion of fifteen amino acid residues. The PCR product including 369aa open reading frame was one of the ten isoforms which had been reported before and it was named MCP-C2.
黄健,李文鑫,苟德明,蒋达和.人MCP cDNA的克隆、序列分析及同种型的比较[J].生物化学与生物物理进展,1999,26(4):355-358
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