人鼻咽与鼻咽癌及肺癌基因表达谱差异的研究
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国家自然科学基金重点项目(39730200)和美国中华医学会基金项目(96-655)资助.


Study of the Gene Expression Profile on Nasopharyngeal Carcinoma,Lung Cancer and Normal Adult Nasopharynx Tissues by High Density Microarray
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    摘要:

    研究鼻咽癌、肺癌与正常鼻咽组织基因表达谱差异及筛选鼻咽癌相关基因,采用α- 32P逆转录标记组织总RNA,将cDNA探针与有5 184个基因或表达序列标签EST(expression sequence tag)的高密度cDNA微阵列GF200杂交,软件分析表达谱差异.结果发现三者均呈低表达为主的表达谱,密度值在200以上的基因及EST在鼻咽癌有110个,肺癌134个而鼻咽组织有158个;5个EST在鼻咽高表达但鼻咽癌低表达,3个EST在鼻咽癌高表达但正常鼻咽低表达.结果表明鼻咽癌与正常鼻咽及肺癌组织存在差异表达基因,可能还有新基因在鼻咽癌发生中起作用;采用高密度cDNA微阵列是一种筛选差异表达基因的快速有效方法.

    Abstract:

    To study the gene expression profile on nasopharyngeal carcinoma (NPC),lung cancer (LC) and normal adult nasopharynx (NP) tissue and to obtain the NPC-related gene, the total RNA extracted from these tissues were retro-transcripted and labeled with α- 32P isotype. The cDNA probes were hybrided to high density cDNA microarray GF200 with 5 184 genes and ESTs,then the signals were analyzed by Pathways software. The results showed that the low density signals were distributed in three types of tissues and the number of genes and ESTs with the density value over 200 was respectively 110,134 and 158 among NPC,LC and NP tissue. The results indicated that the different expression genes existed among NPC, LC and NP tissues and some new genes could play an important role in NPC. The high-density microarray was a rapid and effective method to select the different expression genes.

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何志巍,许亮国,任彩萍,谢鹭,甘润良,姚开泰.人鼻咽与鼻咽癌及肺癌基因表达谱差异的研究[J].生物化学与生物物理进展,1999,26(5):446-450

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  • 收稿日期:1999-04-05
  • 最后修改日期:1999-05-21
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