藻蓝蛋白(PC)具有特征性吸收光谱和荧光光谱,是一种新颖的荧光分子探针.为了获取高纯度PC,经过反复探索研究建立了SephadexG-200、DEAE-SephadexA-25、HA、SephadexG-200的分离纯化程序。此法效果理想:PAGE显示为单条电泳带;纯度值(A615/A280)高达14,突破了国内外报告的最高值.
The phycocyanin (PC) is a kind of fluorescence molecular probe because it has characteristic absorption spectra and fluorescence spectra.To get highly purified phycocyanin,the successive procedure of column chromatography was set up with SephadexG-200、DEAE-SephadexG-25、HA、and SephadexG-200.The results showed that the purity standral (ratio of A615/A280) reaches to 14 at pH 7.0,and it has been demonstrated by PAGE as a single band.
王勇,钱凯先,董强.高纯度藻蓝蛋白分离纯化及光谱特性研究[J].生物化学与生物物理进展,1999,26(5):457-460
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