根据鱼类外周血细胞都有核的特点,采用从冷冻和低渗双重处理分离的细胞核提取基因组DNA.以此法获得的白鲢和鳙鱼的基因组DNA为模板,和Operon公司生产的OPN和OPM两个组共40个随机引物,对这两种鱼进行了随机扩增多态DNA(RAPD)分析;确定了对这两种鱼基因组相关区域可进行随机PCR扩增的有效引物,特别是哪些可产生种群内或群体的RAPD遗传标记,即可产生个体特异性和群体特异性RAPD带谱的引物.讨论了RAPD遗传分子标记在鱼类遗传,特别是遗传多样性研究,和鱼类种质资源评估和管理中的应用前景问题.
Total 40 random primers of OPN and OPM groups made in Operon Co. were applied to the RAPD analysis of H.molitrix and A.nobilis. Besides establishment of species-specific RAPD electrophoretogram, some kinds of primers which can produce molecular genetic markers of RAPD with individuality- or population-specificity of these fish were found. A modified method to extracting genomic DNA from peripheral blood cells of fish, and the application prospects of RAPD technique in genetic diversity and variation of fish, and in fishery management and the evaluation of fish resources were discussed.
张锡元,杨建琪,张德春,邓凤姣,余来宁,方耀林.白鲢和鳙鱼的随机扩增多态DNA分析[J].生物化学与生物物理进展,1999,26(5):469-472
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