用化学发光法研究了邻苯三酚碱性自氧化产生O2-·的发光行为.通过对测定条件的研究, 得出最佳测定方案.改进后的方法不需使用发光剂Luminol, 所用试剂价廉易得, 且方法稳定, 重现性好.此法灵敏度远高于邻苯三酚自氧化的其他文献报道法.
The chemiluminescence behaviour of pyrogallol alkali autoxidation produced O2-· was studied. The optimum conditions were investigated by examining the influence of various factors. The improved method needn't to use the luminous agent——luminol, the reagents used are cheap, both the stability and reproducibility are good, and the sensitivity is far higher than the report of other method of pyrogallol autoxidation.
许申鸿,杭瑚.邻苯三酚-碳酸盐缓冲液化学发光体系的研究[J].生物化学与生物物理进展,1999,26(5):488-491
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