Cloning and Expression of the Cytoplasmic Domain of Human Erythrocyte Band 3 Protein
Author:
Affiliation:
Fund Project:
摘要
|
图/表
|
访问统计
|
参考文献
|
相似文献
|
引证文献
|
资源附件
|
文章评论
摘要:
人红细胞带3蛋白胞质段(cytoplasmic domain of band 3, cdb3)起着将膜与膜骨架、细胞内环境相联系的重要作用. 以带3蛋白全长基因为模板，用PCR方法扩增出cdb3片段，克隆至pRSET表达质粒上，转化大肠杆菌BL21(DE3). 转化菌经诱导表达出较高含量的cdb3蛋白，纯化后，测得对醛缩酶有抑制活性.
Abstract:
The cytoplasmic domain of human erythrocyte band 3 (cdb3) plays an important role in connection of the membrane with the membrane skeleton, and environment inside the cell as well. With whole length of band 3 gene, cdb3 gene was amplified by PCR and then was cloned into pRSET plasmid. The plasmid was introduced into E.coli BL21(DE3). The cdb3 expression proteins were purified and its function was verified according to the ability to inhibit aldolase.
扫码关注