为探讨双位点核酶对乙型肝炎病毒C基因体外转录物的剪切作用,观察双位点核酶对单一核酶体外剪切的增强作用,同时比较串联核酶和混合核酶的体外切割作用,构建了核酶Rz1,Rz3, Rz1和Rz3的串联核酶(Rz13)体外转录载体, 经体外转录后切割靶RNA. 结果表明:双位点核酶,无论是串联或混合核酶均可增强单一核酶的体外切割作用, 串联和混合核酶中的单一核酶可独立发挥作用;当串联和混合数目为2个时,两者的切割效率差别不大(P>0.05).
To detect cleavage activity of anti-HBV two-unit ribozyme and single ribozyme,and compare two conected ribozyme cleavage efficiency with two mixed ribozymes, firstly, Rz1,Rz3 and Rz13 ribozymes transcription vectors were constructed,then cleavage activity of Rz1,Rz3 and Rz13 ribozymes on target RNA were observed. The results showed that antiviral activity of two-unit ribozyme,whether connected (Rz13)or two mixed ribozymes, has higher cleavage efficiency than single ribozyme. Cleavage efficiency has no difference between connected (Rz13) and two mixed ribozymes(n=2,P>0.05).
连建奇,周永兴,金由辛.双位点核酶对乙型肝炎病毒C基因体外转录物的剪切作用[J].生物化学与生物物理进展,1999,26(6):566-569
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