As a good object to observe the level of glucose in the blood,glycohemoglobin（HbA_1c）has been accepted by many clinical laboratory.The method of testing HbA_1c accurately need expensive equipment, not fit to routine laboratory. The micro-column-test(product of BIO-RAD) was used to determine the percentage of HbA_1c in the whole blood of 80 health residents and 65 insulin-dependent diabites meuitus(IDDM) and non-insulin-dependent diabetes (NIDDM). 5 NIDDM samples and 5 IDDM samples were assayed 10 times each in a single test run,the average value of CV was 1.2%,the concentration of HbA_1c was 4.8%,8.9%,15.3%.The average value of the repeat runs was 104.2%.The method does not require special equipment and is simple to do.The result of the test demonstrated that the overall ranges of values for the heath resident tested was 4.6%±2.3%;that for NIDDM samples was 8.3%～15.2%;the value of IDDM samples was 8.5%～22.4%. The level of HbA_1c and the time of maintain is closely associated with DM relative symptom of the patients.