以培养的人肝癌细胞系HepG2为研究对象,采用“冻干浓缩培养液载脂蛋白测定法”,考察了地塞米松对HepG2细胞载脂蛋白(apolipoprotein,apo)AⅠ、AⅡ、CⅢ、B100及E分泌的影响.结果表明:地塞米松对HepG2细胞apoAⅠ和apoE的分泌有促进作用,对apoAⅡ、apoB100和apoCⅢ的分泌有抑制作用,且这种作用随地塞米松浓度的增加而增强.当培养液中地塞米松的浓度为5.5×10-5mol/L时,apoAⅠ和apoE的分泌分别增加36.6%和49.4%(P<0.01),apoAⅡ、apoB100和apoCⅢ的分泌分别减少38.9%、31.9%和29.8%(P<0.01).
In order to obersve the effect of dexamethasone on the secretion of apolipoproteins AⅠ,AⅡ,CⅢ,B100 and E by cultured HepG2 cells. The apolipoprotiens contents in culture media were measured by radioimmuodiffusion assay (RID) kits developed by authors' research unit. 20-fold lyophilizely condensed culture media were used for the assays.The results showed that dexamethasone can increase the secretion of apoC and E, and inhibit the secretion of apoAⅡ,B100 and CⅢ;and the effect of dexamethasone were strengthened in a dose-dependent manner. When the concentration of dexamethasone was 5.5×10-5mol/L in the culture media,the secretion of apolipoprotein AⅠand E increased 36.6% and 49.4%(P<0.01)respectively, while the secretion of apo AⅡ,B100 and CⅢ decreased 38.9%、31.9% and 29.8%(P<0.01)respectively.
刘皓,吴兆丰,刘秉文.地塞米松对HepG2细胞分泌载脂蛋白的影响[J].生物化学与生物物理进展,2001,28(1):77-80
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