机械拉伸对血管平滑肌细胞粘附及生长的影响
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国家自然科学基金重点项目(19732003).


The Effects of the Mechanical Stretch on the Adhesion and Growth of Vascular Smooth Muscle Cells in vitro
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This work was supported by a grant from National Natural Sciences Foundation of China (Stress-Growth Relationship of cells and Living Tissues,19732003).

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    摘要:

    通过自行研制的“四点弯曲梁”实验装置对血管平滑肌细胞(VSMC)加载培养,并结合显微形态观察和计算机图像处理系统测量细胞铺展投影面积、微管吸吮实验系统检测细胞与表面的粘附力、α-肌动蛋白(actin)免疫组化试验,了解细胞骨架发育和排列取向、流式细胞仪检测细胞动力学以及细胞生长行为等认识VSMC对应变刺激的响应.发现VSMC粘附铺展与实验时间正相关,细胞粘附力、铺展面积、单位面积粘附力4 h后实验组与对照组无显著性差异.VSMC内α-actin发育随加载时间延长呈增加趋势.细胞动力学检测实验组加载24 h后VSMC增殖活动受到抑制.VSMC可能通过调节细胞铺展行为、胞内应力纤维发育等主动机制,实现对机械拉伸的适应性改建.应变刺激有利于体外培养的VSMC维持收缩表型.

    Abstract:

    An in vitro model was built for researching the effects of strain on vascular smooth muscle cells (VSMCs). The cultured VSMCs was stretched by four-support-bending-beam system, then the project area of cells was measured by computer-image-processing, the adhesion force was measured by micropipette-aspirating system, the α-actin of VSMCs was distinguished by immunocytochemistry and the dynamic of VSMCs was determined by FCM. The results show that: (1) The adhesion force of VSMCs is positively related to time. The adhesion force of unit area is indistinct after stretched for four hour. (2) The amount of α-actin increases with stretching time. (3) The proliferation of VSMCs is a little inhibited by stretched 24 h. These results suggest that the VSMCs in vitro could adjust their behavious to adapt the tension.

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王红兵,黄岂平,卢晓,秦建,王远亮,蔡绍皙.机械拉伸对血管平滑肌细胞粘附及生长的影响[J].生物化学与生物物理进展,2001,28(1):103-107

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  • 收稿日期:2000-01-16
  • 最后修改日期:2000-03-31
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