This project is supported by 863 foundation. The project is “Rapid determination of BOD by biosensor”.
基于生物催化和免疫原理的生物传感器在环境领域中获得了广泛应用.近年来,随着分子生物学和生物技术的发展,人们开发了以核酸探针为识别元件,基于核酸相互作用原理的DNA生物传感器.该传感器可用于受感染微生物的核酸序列分析、优先控制污染物的检测以及污染物与DNA之间相互作用的研究,在环境污染监测中具有潜在的巨大应用前景.简要介绍了核酸杂交生物传感器的基本原理及其在环境微生物和优先控制污染物(priority pollutant)检测中的应用研究进展.
The biosensors based on biocatalytic and immunosensors have gained increasing applications in environmental biomonitoring. Along with the advances in molecular biology and biotechnology, the DNA biosensors, based on DNA interaction and using nucleic acid as recognition element, was developed. They can be used for the hybridization detection of nucleic acid sequences from infectious microorganisms, for monitoring of priority pollutants and for the interacting study of pollutants and DNA. They possess a great potential for environmental monitoring. The principle of nucleic acid hybridization biosensors and their applications for the detection of environmental microorganisms and the priority pollutants was briefly described.
王建龙. DNA生物传感器在环境污染监测中的应用[J].生物化学与生物物理进展,2001,28(1):125-128
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