P₅₃ gene (exon7～8) mutatins and p53 proteins and HPV 6，11，16，18-DNA were examined in 49 cervical carcinoma by immunohistochemistry, polymerase chain reaction (PCR) and single strand conformation polymorphism (SSCP) in order to investigate their role and mutual relation and clinical significance in the oncogenesis of cervical carcinoma. The results showed that first, p53 proteins positive rate was 48.98％, and not outstandingly related to the differentiation and the invasive degree of cervical carcinoma(P＞0.05); the defects of P₅₃ gene (exon7～8) were not found but P₅₃ (exon7～8) mutations were detected in 7 of 49(14.29％) cervical carcinoma; then, HPV16-DNA positive rate was much higher than HPV6,11,18-DNA positive rate respectively(P＜0.001),and the different HPV-DNA was simultaneously tested in one cervical carcinoma; last, not all cases of P₅₃ mutations had p53 proteins positive, but the cases of P₅₃ mutations and p53 proteins negative certainly had HPV infections, and HPV positive cases were much more than its negative one in the cases of p53 proteins positive(P＜0.001). These results proved that the oncogenesis of cervical carcinoma is mainly associated with HPV16 infections, and second related to P₅₃ (exon7～8) mutations. p53 proteins positive results from P₅₃ mutations or/and HPV infections in cervical carcinoma.