MT and αα genes were introduced into Petunia by Agrobacterium-mediated transformation. Transformants, especially the ones transformed by αα gene, displayed the obviously higher resistance and accumulation of Pb than control plants. The growth of the roots and shoots of plants transformed by MT and αα genes was unaffected by up to 150 μmol/L Pb and 200 μmol/L Pb, respectively; whereas control seedlings showed severe inhibition of roots’ and shoots’ growth and leaves’ wither under 100 μmol/L Pb. After the transformants were selfed, the seedlings of offspring from MT and αα positive plants were tested for resistance to Pb. It was found that the transformants could tolerate higher concentration of Pb. The detection of accumulation of Pb in the transformed plants by MT and αα genes showed 28% and 35% more than non-transformants, respectively. The results of these experiments indicated that the ornamental plant:Petunia could be used for treatment of the toxic heavy metal Pb pollution in soil.