国家自然科学基金(39970236)及国家重点基础研究发展规划项目(G1999054007).
This work was supported by grants from the National Natural Foundation of Sciences (39970236) and National Key Foundation of Development and Research (G1999054007).
用乙醛对人类神经tau进行醛胺化,通过NCLK(neuronal cdc2-like protein kinase)和[γ-32P]ATP对其磷酸化.磷酸化的产物经胃蛋白酶降解及HPLC(C-18)分析降解片段,发现醛胺化tau的降解物中有两个新的磷酸化肽段(A4和A6).
Human neuronal tau-40 was acetylated and then phosphorylated by neuronal cdc2-like kinase (NCLK) with [γ-32P] ATP, and then the phosphorylated acetyl-tau (PAtau) was digested with pepsin. Compared with the phosphorylated tau (Ptau), two new fractions of radioactivity from PAtau were obtained as it was eluted by C-18 column on HPLC.
华茜,聂春来,陈永辉,张岱,赫荣乔.乙醛对人类神经tau磷酸化的影响[J].生物化学与生物物理进展,2001,28(5):761-763
复制生物化学与生物物理进展 ® 2025 版权所有 ICP:京ICP备05023138号-1 京公网安备 11010502031771号