国家自然科学基金(19890300)和安徽省自然科学基金资助项目(00041110).
This work was supported by grants from The National Natural Sciences Foundation (19890300) and Anhui Province Natural Sciences Foundation(00041110).
以水稻品种农林8号及其60Co γ射线辐照突变体农林8号m为研究材料,选用360个10碱基寡核苷酸随机引物,利用随机扩增多态性DNA标记技术筛选出1个引物OPG18在农林8号和农林8号m之间表现出共显性的多态性.通过对该共显性标记的克隆和序列分析表明,突变体农林8号m与农林8号相比有29 bp DNA片段的缺失.研究结果为60Co γ射线辐照导致植物基因组DNA缺失提供了一个最直接明确的证据.
A total of 360 arbitrary 10-mer oligonucleotide primers were screened using RAPD (random amplified polymorphic DNA) technique on the genome DNA of Norin 8 and its 60Co gamma-ray irradiation mutant Norin 8m. One primer OPG18 produced co-dominant polymorphic markers OPG18/972 and OPG18/943 from Norin 8 and Norin 8m. Based on the clones and sequences of the OPG18/972 and OPG18/943, it shows that a 29 bp DNA fragment in the genome DNA of Norin 8m is deleted. The results directly confirmed the fact that 60Co gamma-ray irradiation can induced a bigger DNA fragment deletion of plant genome DNA.
向太和,杨剑波,杨前进,朱启升,李莉,倪大虎,汪秀峰,黄大年.60Co辐照对水稻基因组DNA诱变的分子生物学效应[J].生物化学与生物物理进展,2002,29(5):754-759
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