With the development of the technique of nuclear transplantation, nuclear-transfered embryonic stem cell line of cattle, mice and human-rabbit inter-species now have produced. Although experimented therapeutic cloning in animal showed potentially clinical applications in human being, research of human nuclear-transfered embryonic stem cell is confronted with many problems, such as low efficiency of production of nuclear-transfered embryonic stem cell lines, the limited derivation of oocytes, dispute of ethic and politics, therapeutic security and etc. In the long run, the efforts should be focused on the development of cloning efficiency and the solution of ethical and politics concerns with scientific and idealistic progress. Nuclear-transfered embryonic stem cells will meet the high expectations of human for rejuvenation of the aging or diseased body.