利用有序差异显示技术克隆受稻瘟病菌诱导表达的水稻cDNA的研究
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国家自然科学基金资助项目(30040025)和广东省科技攻关项目(99M01503G).


Application of Ordered Differential Display to Isolate Rice cDNAs Induced by Magnaporthe grisea
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This work was supported by grants from The National Natural Sciences Foundation of China (30040025) and Scientific Technology Research Fund of Guangdong Province (99M01503G).

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    摘要:

    利用有序差异显示技术(ODD)分析受稻瘟病菌诱导表达的水稻基因,通过反式RNA印迹进行辅助筛选,获得了37个在接种稻瘟病菌后表达量增强的水稻cDNA克隆.采用RNA印迹对其中5个克隆在接种稻瘟病菌后的表达分析表明,这些克隆在抗病以及感病的水稻株系中都具有诱导表达的特点.根据序列同源性分析,与这些克隆序列相应的同源基因可能涉及抑制病原菌生长、清除真菌毒素、传递抗病信号以及调节宿主生理状态等几方面的功能.

    Abstract:

    Ordered differential display (ODD) was used to clone differentially expressed genes in rice induced by Magnaporthe grisea. Of 37 cDNA clones initially identified by reverse Northern blot, 5 clones were chosen as probes for further Northern test. The result showed that all of these 5 clones were expressed both in highly, moderately resistant and susceptible rice lines in a pathogen inducible manner. According to the homology analysis, these clones were suggested to be involved in plant defense response by: (1) inhibiting growth of fungus and even causing its death; (2) excluding toxic compounds delivered by fungus; (3) priming signal transduction; (4) and/or regulating metabolic activities in agreement with plant defense.

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罗文永,李晓方,胡骏,刘文华,肖昕,刘良式.利用有序差异显示技术克隆受稻瘟病菌诱导表达的水稻cDNA的研究[J].生物化学与生物物理进展,2004,31(7):635-642

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  • 收稿日期:2004-01-14
  • 最后修改日期:2004-02-05
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