Nuclear transfer has been used to clone several mammalian species，including sheep, goats, cattle, pigs, mice, rabbits, houses, and mules. However, still there are many problems such as low efficiency, high abortion, malformation, huge fetus, high neonatal death need to settle. So it was important to analyze a wide variety of genes in individual cloned embryos. But using conventional RT-PCR strategies permitted analysis of only a few genes in one embryo. A global RT-PCR strategy has been used, allowing the analysis of almost hundreds of genes from a single embryo. This method was not seen used to analyze the genes in cloned embryos. Bovine oocytes have been used to investigate the expression of genes related to development such as IL6, IFτ, CX43, PSMC3, oct4, DNMT1. The results were same as those obtained by using conventional RT-PCR strategies. Further it forth the first time reported the expression of DNMT1 and PSMC3 gene in bovine oocytes.