国家自然科学基金(30170244和10172009)、中国博士后基金(2003034083)和河北省博士基金(B20031158)资助项目.
This work was supported by grants from The National Natural Sciences Foundation of China (30170244, 10172009), The China Postdoctoral Science Foundation (2003034083)and The Hebei Doctoral Foundation (B20031158).
采用 60Co大剂量全身均匀急性辐射的方法,造成一种辐射贫血的动物模型.以便在几天内连续研究 60Co辐射对新生的网织红细胞及红细胞流变学特性的影响.采用一种在低粘切变流场中能将红细胞变形指数DI分解为取向指数(DI)or和小变形指数(DI)d的新型激光衍射法,对网织红细胞及红细胞的变形指数、取向指数、综合变形指数(IDI)等血液流变学特性参数进行测量,发现在 60Co大剂量辐射后,新生的网织红细胞及红细胞流变学特性存在明显异常.将这种 60Co辐射造成的贫血模型与文宗曜等提出的用抗体诱导的大量同步化的球形红细胞贫血模型相比较,后者更具有明显的优点.同时为研究辐射对血液流变特性的影响及正确地挑选红细胞衰老模型提供了理论与实验的基础.
The microrheological characteristics of reticulocytes in vivo was studied. Method of anemia in rabbits induced by 60Co radiation was used. The measurements of percent of reticulocytes, deformation index, orientation index, etc in the processes of reticulocytes changing into erythrocytes in vivo for 72 h were performed. It was shown that there were obvious changes in the reticulocytes rheological characteristics in the courses of this changing. Therefore, the found had some basic theoretical and clinical significance.
谢利德,杨海杰,张卫光,孙大公,文宗曜,谭文长.60Co辐射对网织红细胞微观流变学特性的影响[J].生物化学与生物物理进展,2004,31(7):666-670
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