Three cloned cattle from the same somatic cell line (TT, QQ, XW) and three transferred cloned cattle from the same gene transferred cell line (JM, LW, 8C2), together with two randomly selected cattle, one LUXI (LX) and one Holstein (HS), were genetically analyzed using 24 fluorescent microsatellite markers. There were 1～5 alleles at 24 loci, the average being 3.17. Among TT, QQ, XW, JM, LW, 8C2, the somatic cell line and the gene transferred somatic cell line, the probabilities of matching (P_M) between individuals were 1.17×10^-36 using allele frequencies obtained from public data on internet and 1.90×10^-23 by defining the allele frequencies at each locus as the reciprocal of the number of alleles at that locus. In addition, their genotypes differed from those of the randomly selected LX and HS at 23 and 20 marker loci respectively.