In order to study the biological function and conservation of homology to the human prostate and colon cancer-associated gene (hPC-1), the complete cDNA sequence from mouse kidney was cloned. The gene was named mPC-1 (GenBank ACC No.AY048852), and its cDNA was 2 193 base pairs. The whole cDNA locus was mapped to mouse chromosome 3A1～A2 by BLAST analysis to the mouse genome. The longest ORF of mPC-1 encoded the putative protein of 224 amino acids, which is 82% identical to hPC-1 coding region, with a coiled-coil domain and PEST domain in each. Bioinformatics analyses show that mPC-1 is comprised of six exons and highly homologous to mD 52 and its first exon is a highly special gene sequence thereof. Special promoter of mPC-1 has been verified experimentally, therefore, it was assumed that mPC-1 is a gene overlapping with mouse mD 52. RT-PCR performed in different mouse tissues or organs and various embryogenesis showed that the expression of the gene was strongly in prostate, kidney and eye, slightly low in stomach and smooth muscle, but not or very weakly in other tissues, however, the mD 52 gene almost expressed ubiquitously in mouse tissues or organs. Consequently, the gene sequence between mPC-1 and mD 52 is markedly overlapping in large-scale but modulated independently. Taken together, the results suggest that mPC-1 is a novel gene in coincidence to hPC-1 either structure or function.