In oder to study effect of BRD7 gene on NPC cell line CNE1, BRD7 was introduced into CNE1 cells by liposome transfection. BRD7 transfected cells were resulted in a declined growth curve. To account for the mechanism of this gene function on CNE1, two-dimensional polyacrylamide gel eletrophoresis(2-D PAGE) and MALDI-TOF were performed. After image analysis and MALDI-TOF identification, 19 differential expression proteins were identified. These proteins included BCCIP(BRCA2 and CDKN1A (p21(Waf1/Cip1)), FHL2(four and a half LIM domains 2), Chloride channel regulatory protein, Hin-1 (high-in-normal-1), WISP-1 (connective tissue growth factor related protein), SREC-4(scavenger receptor expressed by endothelial cells-2), folate receptor, which involved in transcription regulation, adherence and so on. The study extended the research field of BRD7 and reinforced the evidences that BRD7 act as a NPC-related candidate suppressor gene.