It has been demonstrated that there are differences between introns of highly-transcribed genes and those of lowly-transcribed genes in sequence length, position preference and oligonucleotide usage. Further observation showed a similar phenomenon: the lengths of upstream intergenic sequences of highly-transcribed genes are generally longer than those of lowly-transcribed genes. Based on the statistical comparative analysis on the occurrence frequencies of oligonucleotides in the upstream intergenic regions of the two sets of genes, some potential sites were extracted in the upstream regions of highly-transcribed genes which are likely to enhance the transcription of genes. These regulatory elements turned out to be also over-represented by comparing the upstream sequences of highly-transcribed genes to all non-coding sequences of yeast genes. Most of these elements are agreement with transcription factor binding sites obtained from experimental analyses. And these elements are G,C rich, this seems to be supplement to those potential sites in introns extracted before, which are A,T rich, in base composition. Such sequence structures of highly-transcribed genes are favorable to the transcription of genes.