Using bisulphate sequencing, methylation patterns of the Xist gene in cloned blastocysts derived from bovine fetal fibroblasts (FFB) or oviduct epithelial cells (FOV) were investigated, as compared to that in donor cells and in vitro fertilized (IVF) blastocysts. Hypomethylation of the Xist gene was observed in the cloned blastocysts (43% and 17% in FFB and FOV derived blastocysts, respectively), while the Xist gene in 49% IVF blastocysts appeared to be methylated. On the contrast, somatic donor cells showed high percentage of methylation (66% and 63% in FFB and FOV cells, respectively). These results suggest that the DNA methylations of the Xist gene were reprogrammable and the detected CpG islands may regulate expression of the Xist gene. Altogether with the previous data, It was shown that development rate of cloned blastocysts derived from FFB is lower than that from FOV of the same origin, while pregnancy and birth rates appear to be opposite. It can be proposed that cloned blastocysts derived from different types of somatic cells differ in DNA reprogramming that may affect embryo and fetal development.