国家自然科学基金资助项目(30370030).
This work was supported by grants from The National Natural Science Foundation of China (30370030).
通过构建嗜水气单胞菌AH-1 Quorum Sensing (QS) 2个关键调节基因ahyI,ahyR的突变菌株,来系统分析嗜水气单胞菌AH-1Ⅲ型分泌系统基因,揭示它们由QS系统调控. 在ahyI突变菌中,TTSS分泌效应因子(effector) aexT 量显著提高.通过构建LacZ-TTSS基因启动子融合表达,进一步表明QS系统负调控编码TTSS 组分的基因.
A systematic analysis of the typeⅢ secretion genes of Aeromonas hydrophila strain AH-1 by constructing ahyR and ahyI mutant revealed that they are under quorum-sensing control. This observation was supported by the down-regulation of the TTSS genes in the presence of lacZ-TTSS gene promoter and the corresponding advanced secretion of AexT in ahyI mutant.
周文广,刘广锋,周世宁.嗜水气单胞菌AH-1 quorum sensing 负调控Ⅲ型分泌系统的表达[J].生物化学与生物物理进展,2007,34(6):647-652
复制生物化学与生物物理进展 ® 2025 版权所有 ICP:京ICP备05023138号-1 京公网安备 11010502031771号