国家自然科学基金(30370736, 30570966)、教育部博士点基金(20050159023)和教育部回国人员科研启动基金资助项目.
This work was supported by grants from The National Natural Science Foundation of China (30370736, 30570966), Doctoral Fund of Ministry of Education of China (20050159023) and Scientific Research Foundation for Returned Scholars,Ministry of Education of China.
研究p21活化激酶2 (p21-activated kinase 2,PAK2) 在卵母细胞成熟过程中的作用. 以爪蟾卵母细胞为模型,分别向爪蟾卵母细胞显微注射PAK2-N端(PAK2-N-terminal,PAK2-NT)和PAK2-N端突变体(PAK2-N-terminal mutation,PAK2-NTm) mRNA,荧光显微镜下观察胚泡破裂发生. 采用共聚焦显微镜,时间延迟摄影法观察正常卵母细胞、PAK2-NT mRNA注射组和PAK2-NTm mRNA注射组卵母细胞胞质分裂、极体形成及与Cdc42活性的关系. 结果表明,PAK2-NT mRNA和PAK2-NTm mRNA注射组的卵母细胞与正常卵母细胞胚泡破裂发生相似,但PAK2-NT mRNA和PAK2-NTm mRNA注射组未见胞质分裂和极体形成. 结果提示,PAK2参与卵母细胞胞质分裂和极体形成可能不依赖于Cdc42的调节过程.
p21-Activated kinases including p21-activated kinase 2 contributed to the regulation of actin cytoskeleton and cell dynamics. In order to investigate the function of PAK2 on the maturation of Xenopus oocyte, PAK2-NT(PAK2-N-terminal,PAK2-NT) and PAK2-NTm (PAK2-N-terminal mutation) mRNA were microinjected into Xenopus oocyte respectively. Under fluorescent microscopy germinal vesicle breakdown was observed during cytokinesis. To further observe the relationship of oocyte cytokinesis, polar body formation and Cdc42 activity, confocal microscopy with time-lapse was employed . As a result, occurrences of germinal vesicle breakdown in oocytes were similar to those oocytes injected with PAK2-NT mRNA or injected with PAK2-NTm mRNA,but no cytokinesis and polar body formation were observed in oocytes injected with PAK2-NT mRNA or PAK2-NTm mRNA. These results indicated that PAK2 involved in Xenopus oocytes cytokinesis and polar body formation independent of Cdc42 activity.
程大也,梁彬,李丰.爪蟾p21活化激酶2参与卵母细胞胞质分裂过程不依赖于Cdc42[J].生物化学与生物物理进展,2007,34(6):653-658
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