The investigations on the interactive relationships of DNA replication, damage repair and recombination have been locating in the frontline and becoming one of the hotspots in today's life science research. More and more studies show that the processes of DNA replication, damage repair and recombination are both independent and interdependent in the molecular level. These pathways coordinate and conform each other through interactions of many critical proteins in the pathways, by which DNA molecules, known as genetical materials, can be well maintained in cell and faithfully transferred through cellular generations. By using E. coli as a model system, the recent progresses and the possible rules underlying E. coli DNA replication, repair and recombination have been analyzed. As it is believed that the researches on E. coli DNA replication, repair and recombination may be capable of providing clues to the eukaryotic research based on the universal conservations of the critical proteins in the pathways.