Somatic cell nuclear transfer (SCNT) has great value in medicine, stock breeding and saving endangered animals, but the low efficiency of SCNT restricts its application. Imprinted genes regulate fetal growth and many are essential for normal development in mammals. GTL2 is imprinted in human and mouse, which act as noncoding RNAs regulating the translation of target mRNA. In order to identify the expression of GTL2 in cattle produced by natural reproduction and by SCNT, a single nucleotide polymorphisms (SNPs) in GTL2 through PCR-SSCP was identified and the GTL2 expression patterns in six organs of hybrids was analyzed by RT-PCR-SSCP. The results demonstrated that GTL2 was monoallelic expression in all six examined organs of cattle produced by natural reproduction, and showed monoallelic expression in heart and liver but biallelic expression in brain, spleen, lung and kidney of cattle produced by SCNT that died shortly after birth. The abnormal expression of GTL2 may contribute to the organ development defects and the low efficiency of SCNT.