国家科技重大专项资助项目(2008ZX08012-003),国家科技支撑计划资助项目(2006BAK04A02)
This work was supported by grants from The National Key Scientific and Technological Project (2008ZX08012-003) and The National Science and Technology Supporting Plan (2006BAK04A02 )
综述了ICP-MS法应用于蛋白质定量技术方面的研究进展.蛋白质定量研究已成为蛋白质组学研究领域的热点,它是解析生物体蛋白质功能的重要途径.基于同位素标记和生物质谱分析技术是蛋白质定量最常用的方法之一,近年来,随着质谱技术的发展,电感耦合等离子体质谱( ICP-MS) 技术成为元素测量的重要手段,这使其在蛋白质定量中具一定的应用前景.
Protein quantitative research has become a hot topic in proteomics and a main approach to discover biological protein functions. The technique based on isotope labeling and biological mass spectrometry analysis is one of the most commonly used strategies for protein quantification. In recent years, with the development of mass spectrometry techniques, inductively coupled plasma mass spectrometry (ICP-MS) becomes a powerful tool for research and measurement of elements, and may be enabling new insights and broad application in protein quantification. The advance of ICP-MS based techniques in the research of protein quantification was reviewed.
米薇,王晶.基于电感耦合等离子体质谱的蛋白质定量技术研究进展[J].生物化学与生物物理进展,2010,37(2):224-229
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