国家高技术研究发展计划(863)(2007AA042106), 国家重点基础研究发展计划(973)(2007CB714502, 2007CB936000), 卫生部重大专项(2009ZX10004-301, 2009ZX10004-505), 江苏省医学重点建设学科资助(XK200723), 南通市社会发展计划(S8919, S2009012)资助项目和上海市科委项目(08110700200, 0752nm021)
This work was supported by grants from Hi-Tech Research and Development Program of China (2007AA042106), National Basic Research Program of China (2007CB714502, 2007CB936000), Ministry of Health (2009ZX10004-301, 2009ZX10004-505), Key Subject of Jiangsu Province (XK200723), Society Development Foundation of Nantong (S8919, S2009012) and Science and Technology Commission of Shanghai Municipality (08110700200, 0752nm021)
描述了一种微流控芯片电泳快速分离血清高密度脂蛋白(high density lipoprotein, HDL)亚类的方法.利用自制的微流控芯片,结合激光诱导荧光检测系统,40 mmol/L Tricine、50 mmol/L甲基葡胺(MEG)、0.2 mmol/L SDS (pH 8.5)为样品缓冲液,40 mmol/L Tricine、50 mmol/L MEG、0.01 mmol/L SDS (pH 8.5)为分离缓冲液,4 min内HDL3和HDL2两种亚类得到基线分离.该法操作过程简单,重复性较佳,测试费用低廉,在临床HDL亚类的检测中具有较好的应用前景.
A method that could separate the subclasses of high density lipoprotein (HDL) rapidly by microfluicic chip electrophoresis was reported. Combined with laser induced fluorescence detection system, using the microfluicic chip designed independent, using 40 mmol/L Tricine, 50 mmol/L MEG and 0.2 mmol/L SDS (pH 8.5) as sample buffer solutions, using 40 mmol/L Tricine, 50 mmol/L MEG and 0.01 mmol/L SDS (pH 8.5) as separation buffer solutions, the two subclasses, HDL3 and HDL2 could be separated in 4 min. HDL subclasses could be separated with high efficiency and good reproducibility. The operation was easy and cost little. This method could meet the demand of the clinical examination of HDL subclasses, with good clinical value.
郑慧斐,丛辉,王惠民,金庆辉,赵建龙.微流控芯片电泳分离血清高密度脂蛋白亚类的研究[J].生物化学与生物物理进展,2010,37(2):218-223
复制生物化学与生物物理进展 ® 2025 版权所有 ICP:京ICP备05023138号-1 京公网安备 11010502031771号