Previous pharmacological studies found that a water-soluble extract (C-MED-100^TM) of Uncaria tomentosa has antioxidant activity and beneficial effects on DNA repair and immune function. Batch-2 is a novel aqueous extract from cat's claw, however, the free radical scavenging ability and neuroprotective effect of batch-2 have not been reported. Firstly, the neuroprotective effect of batch-2 on the 6-OHDA-induced SH-SY5Y cell damage was detected. Secondly, the component analysis of batch-2 was tested by infrared spectroscopy, HPLC and spectrophotometry.The results showed that batch-2 has scavenging ability to several kinds of free radicals, especial the hydroxyl radical (25 mg/L of batch-2 can scavenge 60% of the hydroxyl radical). The dose dependently attenuated 6-OHDA-induced cell death, lipid peroxidation, mitochondrial potential loss and increase of intracellular reactive oxygen species (ROS) and nitric oxide (NO) in SH-SY5Y cells were also observed. Meanwhile, batch-2 can suppress the 6-OHDA induced increase of expression of iNOS and activity of NF-κB. The results show that the neuroprotective effect of batch-2 on 6-OHDA-induced damage of SH-SY5Y cells by scavenging ROS and NO, inhibiting activation of iNOS and NF-κB. Component analysis shown that the content of polyphenol and quininic acid from Batch-2 was 6.43% and 0.0958% respectively. These results provided evidence to support that the mechanism of batch-2 for antioxidative activity and neuroprotective effect may be similar with (-)-epigallocatechin-3-gallate(EGCG) partly. It is obvious that batch-2 is a potential neuroprotective antioxidant for the precaution candidate of Parkinson's disease.