Metabolic diseases including obesity and diabetes are emerging as major health threats over the world. Cidea, a member of CIDE family proteins, plays an important role in energy homeostasis in brown adipose tissue by regulating lipid storage and AMPK stability. Cidea is tightly regulated at both transcriptional and posttranslational levels, but little is known about its translational regulation. Here, a novel N-terminal truncated isoform of Cidea (mCidea-22) was identified in mouse brown adipose tissue. Using mutational analysis, it was demonstrated that this isoform was generated by alternative translation. Ectopic expression of mCidea cDNA in various cell lines showed that expression of mCidea-22 was cell type specific with its highest expression in preadipocyte 3T3-L1 cells. In addition, it was observed that mCidea-22 had a short half-life and was localized to ER and lipid droplets. Taken together, the data provide strong evidence that translational control played an important role in the regulation of Cidea expression.