In order to study the effects of extracellular matrix (ECM) protein micropatterns on biomaterials surfaces fabricated by microcontact printing (μCP) on human chondrocytes adhesion, spread and protein expression, the bone morphogenetic protein 2 (BMP-2) was printed onto polystyrene (PS) surface to fabricate the ECM protein micropatterns by μCP. Three kinds of excellent micropattern were obtained successfully according to the fluorescent images. The human chondrocytes were seeded on PS (control samples), BMP-2 coated PS surface and PS surface with BMP-2 micropatterns, respectively. As compared with control samples, the human chondrocytes on BMP-2 coating exhibited better growth and spread behavior, at the same time, the BMP-2 coating also cannot promote expression of collagenⅡ and Ⅵ. On the other side, the results concerning BMP-2 micropattens indicated that the protein micropattern surfaces have significant influence on cell adhesion, spread, alignment and functions. The cells preferentially adhered on micropattern protein zones. The micropattern shape and its sizes affected not only the cell adhesion morphology and spread degree but also the typeⅡ and Ⅵ collagen expression. The spread behavior of cell has some positive connections with protein expression and the cell with more spread expressed more typeⅡ and Ⅵ collagen. These results suggested that the ECM protein micropatterns can effectively regulate human chondrocytes growth and functions.