国家重点基础研究发展计划资助项目(973)(2010CB945300)
This work was supported by a grant from National Basic Research Program of China (2010CB945300)
microRNAs (miRNAs)是一类具有转录后调控作用的非编码RNA,在发育、细胞增殖、凋亡及肿瘤发生等多种生理和病理过程中发挥重要作用.为全面了解小鼠B细胞中miRNAs的表达模式,利用流式细胞仪(FACS)分选处于不同发育时期的B细胞,采用TaqMan®低密度芯片对其进行检测,筛选到pre-B阶段9个miRNAs表达量显著上调.将筛选出的miRNAs进行靶基因预测,并对预测靶基因进行功能聚类和通路分析,发现约4%的基因参与免疫系统过程,包括Bcl2、Kit等.选取foxO1与miR-19b、miR-142-3p、miR-106b、miR-182及miR-133b进行初步功能验证,双荧光素酶报告系统及Western blot检测结果均显示,miR-133b可直接作用于foxO1 3′UTR从而降低foxO1的表达.结合人类和小鼠B细胞中foxO1的表达情况分析,其表达模式同miR-133b表达模式呈负相关,说明miR-133b可能参与了B细胞发育过程中foxO1的表达调控过程.
MicroRNAs(miRNAs) are a class of small non-coding RNAs that regulate gene expression at post-transcriptional level. They play important roles in multiple physiological and pathological processes, including development, cell proliferation,apoptosis,metabolism and tumorigenesis, etc. Mouse B cell at different development stages were isolated by FACS and analyzed the miRNAs profile using TaqMan® Low Density Array. The data showed that 9 miRNAs were significantly up-regulated in the pre-B cells. Functional clustering and pathway analysis of 1102 predicted target genes of these miRNAs showed that about 4% of the genes involved in immune system processes, including Bcl2, Kit, etc. A dual luciferase reporter system and Western blot were used to validate the interaction between foxO1 and miR-19b, miR-142-3p, miR-106b, miR-182, miR-133b. The results show that miR-133b can directly regulate the expression of foxO1. According to the foxO1 expression profile of human and mouse, the expression pattern is negatively correlated with that of miR-133b, indicating that miR-133b may be involved in the regulation of foxO1 in B cell development.
梁婧文,王鹏,陈黎,胡益清,孙亿. miR-133b可能通过调控foxO1的表达影响小鼠B细胞发育[J].生物化学与生物物理进展,2011,38(8):744-750
复制生物化学与生物物理进展 ® 2025 版权所有 ICP:京ICP备05023138号-1 京公网安备 11010502031771号