Effect of oxymatrine on the H₂O₂ of L₆ rat myoblast cells was investigated in this study. The hydrogen peroxide(H₂O₂) was used to establish the H₂O₂-induced apoptosis model by L₆ rat myoblast cells that were treated with 0.3, 0.15, 0.75 g/L oxymatrine. The survival rate was measured by MTT method，cell cycles and apoptosis rate of L₆ rat myoblast cells were detected by flow cytometry, the injury degree was determined by apoptosis cytochemistry fluorescent antibody of Bcl-2 and Bax, DAPI staining and HE staining. The protein differences were examined by Western blot. Results showed that survival rate of L₆ myoblast cells was degraded and the apoptosis rate was increased by damaging H₂O₂. The survival rate of L₆ myoblast cells was heightened by oxymatrine of each dose. They could induce the increase in expression of Bcl-2 and the decrease in expression of Bax. Its degree of protection was increased with raising the dosage of oxymatrine and the protective effect of 0.3 g/L dosage is obvious. The protective effect of 0.15, 0.75 g/L oxymatrine were secondary. The main physiological and biochemical effects of oxymatrine was related to protect rat myoblast apoptosis model through NFκB signal transduction. These results implied that oxymatrine may have the potential as a new antioxidant treatment medicine.