To investigate the effects of lentivirus on the growth and development of porcine fetal fibroblasts (PFBs), in this study PFBs were repeatedly infected by lentivirus. The results showed that lentivirus-mediated enhanced green fluorescent protein (EGFP) had stable and efficient expression in PFBs. Under the condition with leukemia inhibitory factor (LIF) and basic fibroblast growth factor (bFGF), some PFBs gradually changed their fibrous growth pattern into round cell morphology. These round cells proliferated and formed cell clones with clear edge boundary. Clones grew rapidly on feeder layers and passaged stably with normal karyotypes. These cells were positive for alkaline phosphatase (AP) and expressed stem cell markers Oct4, Nanog, and SSEA1. In addition, these cells formed embryoid bodies (EB) in vitro and three germ layers in vivo. After the cells were used as nuclear donors, the cleavage rate of the cloned embryos was 53.33%, the morula rate 9.03%, the blastocyst rate 2.07%, and the total cell number per hatched blastocyst was 26.5. Compared with embryos cloned from non- lentivirus PFBs, the morula rate and blastocyst rate were lower and significantly different (P < 0.05). Lentivirus can result in the generation of porcine iPS cells from PFBs, so it can be used as ideal material and tool for research such as epigenetic modification and cell reprogramming.