上海交通大学系统生物医学教育部重点实验室,上海交通大学系统生物医学教育部重点实验室
国家重点基础研究发展计划(973)(2010CB529205)和国家自然科学重大研究计划培育项目(91229108)资助
Key Laboratory of Systems Biomedicine Ministry of Education,Shanghai Jiao Tong University,Key Laboratory of Systems Biomedicine Ministry of Education,Shanghai Jiao Tong University
This work was supported by grants from National Basic Research Program of China (2010CB529205) and The Major Research Plan of National Natural Science Foundation of China(91229108)
单细胞转录组分析以单个细胞为特定研究对象,提取mRNA进行逆转录、放大和高通量测序分析,能揭示该细胞内整体水平的基因表达状态和基因结构信息,准确反映细胞间的异质性,深入理解其基因型和表型之间的相互关系,在发育生物学、基础医学、临床诊断和药物开发等领域都发挥重要作用.本文主要介绍了单细胞转录组分析的特点和技术发展历史以及常用研究策略和不同技术的优缺点,并就其面临挑战和未来发展前景进行了讨论,为该技术的进一步研究与应用提供参考.
Analysis of the transcriptomes of single cells can provide a comprehensive understanding of the heterogeneous expression and gene regulatory networks within a population of cells. Through mRNA isolation, reverse transcription, amplification and deep-sequencing analysis, the transcriptional landscape can be obtained for subsequent detailed quantitative investigation of the levels and variation of gene expression underlying the relationship between the genotype and phenotype of a single cell. Not surprisingly, it has been applied within many fields such as developmental biology, basic medicine, clinical research and drug discovery. This review introduces the history, progress, and technological characteristics of whole transcriptome analyses of single cells, as well as detailed strategies and advantages of the methodology. The technical challenges, potential applications, and future developments are also discussed.
周超平,李鑫辉.单细胞转录组研究进展[J].生物化学与生物物理进展,2013,40(12):1193-1200
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