多途径介导的小胶质细胞间钙波传递
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南开大学物理科学学院/泰达应用物理学院,南开大学物理科学学院/泰达应用物理学院,南开大学物理科学学院/泰达应用物理学院,南开大学物理科学学院/泰达应用物理学院,南开大学物理科学学院/泰达应用物理学院,南开大学物理科学学院/泰达应用物理学院

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国家自然科学基金(11204142),国家重点基础研究发展计划(2010CB934101),高等学校博士学科点专项科研基金(20110031120004),国家基础科学人才培养基金(J1103208)和高等学校学科创新引智计划(B07013)资助项目


Multi-Pathways-Mediated Propagations of Intercellular Calcium Waves in Microglial Cells
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School of Physics and TEDA Applied Physics School, Nankai University,School of Physics and TEDA Applied Physics School, Nankai University,School of Physics and TEDA Applied Physics School, Nankai University,School of physics and TEDA Applied Physics School, Nankai University,School of Physics and TEDA Applied Physics School, Nankai University,School of Physics and TEDA Applied Physics School, Nankai University

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This work was supported by grants from The National Natural Science Foundation of China (11204142), The National Basic Research Program of China (2010CB934101), Specialized Research Fund for the Doctoral Program of Higher Education (New Teachers) (20110031120004 ), The National Science Fund for Talent Training in Basic Sciences (1103208) and 111 Project (B07013)

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    摘要:

    利用微局域机械力刺激,快速实时观察机械力引起的细胞间钙波传递,系统地研究了BV-2小胶质细胞间钙通讯机制.结果表明,在细胞种植密度较小且彼此未接触的情况下,旁分泌途径可介导BV-2小胶质细胞间钙波传递.在细胞密度较大且相互接触的情况下,旁分泌和间隙连接两种途径可共同介导胞间钙波传递.更为有趣的是,在体外发现BV-2小胶质细胞间存在通道纳米管类似物连接,也可介导小胶质细胞间钙波传递.综上所述,小胶质细胞间钙波传递可通过旁分泌、间隙连接和通道纳米管类似物连接三种途径介导.

    Abstract:

    In the present work, we applied mechanical stimulus to study the propagations of intercellular calcium waves among BV-2 microglial cells by the fast fluorescence microscopy imaging system in vitro. The results showed that calcium signaling responded to mechanical stimulus and propagated to neighboring cells in isolated BV-2 microglial cells, suggesting that the paracrine pathway mediated intercellular calcium waves. Further data indicated that gap junction was involved in mechanically-induced intercellular calcium waves when the cells had physical contact with each other. More importantly, tunneling nanotubes (TNTs)-like structure between BV-2 microglial cells was found in vitro and mediated the propagation of intercellular calcium wave. Taken together, our studies demonstrated that three distinct pathways, including paracrine, gap junction and TNTs-like structures, could mediate the intercellular calcium waves in BV-2 microglial cells.

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吴限,潘雷霆,刘颖,邢梦溪,张心正,许京军.多途径介导的小胶质细胞间钙波传递[J].生物化学与生物物理进展,2014,41(2):179-184

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历史
  • 收稿日期:2013-01-04
  • 最后修改日期:2013-07-24
  • 接受日期:2013-07-30
  • 在线发布日期: 2014-02-21
  • 出版日期: 2014-02-20