Adaptation to sensory inputs in animals influences their awareness and reactions to environment changes. The attenuated activities at all of the levels in sensory pathways, such as sensory receptors, afferent nerves and central nervous system, are likely associated with sensory adaptation. The signal encodings in cortical network neurons and astrocytes for sensory adaptation remain unclear. With the methods of two-photon cellular calcium imaging, electrophysiology and pharmacology in mice, we analyzed the dynamics of the barrel cortical neurons and astrocytes in response to repetitive inputs from whisker tactile. The paired stimuli in identical features to the mouse whiskers induced the activities of neurons and astrocytes in the barrel cortex to be spatially attenuated and temporally asynchronized. The interaction between these neurons and astrocytes became less coordinated. The downregulation in the spatial and temporal activities of the neurons and astrocytes was significantly reversed by locally using an inhibitor of glutamatergic AMPA-receptor(AMPAR) desensitization. Therefore, the inactivity and asynchrony of network neurons and astrocytes in barrel cortex through AMPAR desensitization are associated with encoding the adaptation of whisker tactile sensation.