中国海洋大学 海洋生命学院,中国海洋大学 海洋生命学院,中国海洋大学 海洋生命学院,青岛大学 医学院附属医院 山东青岛,中国海洋大学 海洋生命学院
国家科技部国际合作重点项目(2013DFG32880), 国家自然科学基金(31000423)和教育部博士点基金(20120132110012)资助项目
College of Marine Life Science, Ocean University of China,College of Marine Life Science, Ocean University of China,College of Marine Life Science, Ocean University of China,Department of Plastic Surgery, The Affiliated Hospital of Medical College Qingdao University,College of Marine Life Science, Ocean University of China
This work was supported by grants from International S&T Cooperation Program of China (2013DFG32880), The National Natural Science Foundation of China (31000423) and Doctoral Fund of Ministry of Education of China (20120132110012)
喜树碱衍生物对瘢痕疙瘩成纤维细胞的增殖有明显的抑制作用,为提高药物利用度,提供透皮给药的治疗途径,本文采用微乳法制备了一种新的O/W型透明质酸纳米载体(HA-GMS),包载药物10, 11-亚甲二氧基喜树碱(MD-CPT),制备过程中无酒精,且没有使用化学增强剂,经透射电镜和激光粒度仪分别测得HA-GMS纳米乳粒径为(177.33±27.11) nm,zeta电位为-15.6±1.7,多分散系数为0.55±0.01,纳米微乳对MD-CPT药物的包封率为(77.85±1.29)%,且稳定性良好.用MTT法检测HA-GMS对人正常细胞HSF、HUVECs的作用,细胞相对存活率为75%~95%,生物相容性良好,用HA-GMS对瘢痕疙瘩成纤维细胞培养48 h,其生长抑制率为28.2%.HA-GMS纳米乳运载的MD-CPT皮肤渗透量明显高于对照组MD-CPT乙醇溶液对皮肤的渗透量,作用4 h的累积渗透量分别为(660.72±20.54) μg/cm2和(102.73±13.81)μg/cm2,HA-GMS纳米乳明显增加了MD-CPT的透皮效率,本研究结果为透皮给药治疗瘢痕疾病提供了基础.
Camptothecin derivatives have an obvious inhibitory effect on keloid fibroblasts proliferation. In order to improve the utilization rate of drug and provide a transdermal drug treat method, a non-alcoholic hyaluronic acid O/W type nanoemulsion without any chemical enhancers was made to encapsulate 10,11-methylenedioxycamptothecin (MD-CPT). We used transmission electron microscopy (TEM) and laser particle size instrument to survey the HA-GMS nanoemulsion morphology observation, and measured the particle size of (177.32±27.11) nm, the zetaelectric potential of -15.6±1.7, the PdI of 0.55±0.01 and the encapsulation efficiency of (77.85±1.29)%. Determination by MTT method was used to research the effect of biomaterial HA-GMS on HSF cells and HUVECs cells, and the relative cell viability range from 75%~95%, having good biocompatibility, and for keloid fibroblasts culture, growth inhibition rate was 28.2%.The transdermal effect of HA-GMS nanoemulsion carrying MD-CPT was obviously better than that of the control group (CPT MD-CPT ethanol solution), treating for 4 h, the cumulative drug permeation was (660.72±20.54) μg/cm2 and (102.73±13.81) μg/cm2 respectively, the HA-GMS nanoemulsion significantly increased the efficiency of MD-CPT through skin, which may provide a good drug delivery approach.
高媛媛,孔明,程晓杰,王志国,陈西广.载MD-CPT透明质酸纳米微乳经皮给药作用于瘢痕修复的研究[J].生物化学与生物物理进展,2014,41(2):202-208
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