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核酸适配体生物传感器对心肌肌钙蛋白Ⅰ的检测研究
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天津科技大学,天津科技大学,天津科技大学,天津科技大学,天津科技大学,天津科技大学,天津科技大学,天津科技大学

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科技部项目(2013DFG52490), 天津市科委项目(12ZXCXSY07500)和国家自然科学基金资助项目(21076157)


The Detection of cTnⅠ by The Aptamer Biosensor
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College of Bioengineering,Tianjin University of Science Technology,Tianjin Key Laboratory of Industrial Microbiology,Ministry of Education Key Laboratory of Industrial Microbiology,College of Bioengineering,Tianjin University of Science Technology,Tianjin Key Laboratory of Industrial Microbiology,Ministry of Education Key Laboratory of Industrial Microbiology,College of Bioengineering,Tianjin University of Science Technology,Tianjin Key Laboratory of Industrial Microbiology,Ministry of Education Key Laboratory of Industrial Microbiology,College of Bioengineering,Tianjin University of Science Technology,Tianjin Key Laboratory of Industrial Microbiology,Ministry of Education Key Laboratory of Industrial Microbiology,College of Bioengineering,Tianjin University of Science Technology,Tianjin Key Laboratory of Industrial Microbiology,Ministry of Education Key Laboratory of Industrial Microbiology,College of Bioengineering,Tianjin University of Science Technology,Tianjin Key Laboratory of Industrial Microbiology,Ministry of Education Key Laboratory of Industrial Microbiology,College of Bioengineering,Tianjin University of Science Technology,Tianjin Key Laboratory of Industrial Microbiology,Ministry of Education Key Laboratory of Industrial Microbiology,College of Bioengineering,Tianjin University of Science Technology,Tianjin Key Laboratory of Industrial Microbiology,Ministry of Education Key Laboratory of Industrial Microbiology

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This work was supported by grants from the Ministry of Science and Technology of China (2013DFG52490), Tianjin Municipal Science and Technology Commission (12ZXCXSY07500), The National Natural Science Fundation of China (21076157)

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    摘要:

    核酸适配体生物传感器是利用固定在电极表面的适配子与被测溶液中心肌肌钙蛋白Ⅰ(cTnⅠ)发生特异性结合，从而达到检测的目的．我们对玻碳电极进行阳极氧化、氨基化修饰，通过碳二亚胺盐酸盐(carbodiimide hydrochloride，EDC)、N-羟基琥珀酰亚胺(N-hydroxysuccinimide，NHS)活化作用将适配子结合在电极表面．cTnⅠ最佳检测范围是0.05～5 nmol/L，最低检测限为0.05 nmol/L，检测时间为5 min．

    Abstract:

    A new aptamer biosensor for detection of cTn I was established. It can detect cTn I in samples. The surface of GC was modified with anode-oxidation, amidation and activation modification. We used EDC and NHS as activators. Due to aptamer's specificity for cTn I, we can detect the concentration of cTn I in sample solution. The linear range of the analytical signal is observed from 0.05～5 nmol/L. The lowest detected concentration is 0.05 nmol/L. The detection time is 5 min.

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蒋树海,范婷,刘利娟,陈怡,张晓清,沙作良,刘玉良,张娟琨.核酸适配体生物传感器对心肌肌钙蛋白Ⅰ的检测研究[J].生物化学与生物物理进展,2014,41(9):916-920

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  • 收稿日期:2013-09-30
  • 最后修改日期:2013-12-23
  • 接受日期:2013-12-30
  • 在线发布日期: 2014-09-22
  • 出版日期: 2014-09-20
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