Glucose metabolism is one of essential biochemical processes for the survival of living species. In mammals, glucose is utilized in different ways among the different types of cells under different situations. The cells under plenty of oxygen can use oxidative phosphorylation during aerobic respiration. Under hypoxic condition, cells repress oxidative phosphorylation and ferment glucose to lactate. However, some cells choose “Warburg effect”, an activated glycolytic flux even under normoxia, as a way to metabolise glucose. It is known that Warburg effect is favored by tumor cells. Recently, accumulating evidences show that Warburg effect is also involved in mammalian reproduction. Here we summarize the advances on Warburg effect in mammalian reproduction and fertility-related diseases.